FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13627857
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA35750.018.0035048
ACTGAAC36750.017.9749581
CAATGGA50750.017.8958648
CTGCCTA45900.017.686481
TGCTACC48550.017.5863571
CGATTGA44250.017.5390381
CTGATCC36250.017.3827251
GGGACCT33350.017.3512341
AGCTTGA36400.017.3399431
CGGACTG36950.017.3376391
ACTGATG38650.017.3358751
CCGGGAT44800.017.3002721
TATGCCC44400.017.243251
CCGTACT51250.017.151661
GACCTGG41650.017.1462251
TGATTTC33850.017.0949361
TTTACTG47200.017.04361
GGTATTG49200.017.0338331
GGAATAA48100.016.964871
GAATGAA35950.016.8850981
Produced by FastQC (version 0.11.5)