FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15268198
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC39850.017.946741
CTGATCC39000.017.5569741
CGATTGA52850.017.5263021
TGCTACC55100.017.4967671
CTGCCTA47800.017.4445321
AGCTTGA46400.017.4276751
TTTATGC50900.017.4137131
CACCGAA42500.017.3676348
ATGCGAT45250.017.3599991
CAATGGA58650.017.3472258
TATGCCC47000.017.3169171
GGAACTT47800.017.1808871
GGTATTG53550.017.12071
GGGACCT38750.017.0740131
TCGAGGC58400.017.0115931
CCCTCCT65100.016.9546361
CCACCTA60200.016.9390661
CCGGGAT48600.016.8980751
TCTGGAA39600.016.8931641
CGGACTG41050.016.8592781
Produced by FastQC (version 0.11.5)