FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14873656
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACC51950.018.0121571
CACCGAA39000.017.9031058
CGGACTG38700.017.8019051
CCGGGAT46350.017.7866231
CAATGGA55250.017.7684968
ACTGAAC40000.017.7221891
GGATTAC34650.017.6094761
GGTATTG53900.017.5163861
ACTGATG41500.017.4106081
TATGCCC48250.017.3474061
CATCGAA53150.017.21031
TTTACTG46750.017.2076171
CTGCCTA48500.017.1713731
TCGAGGC53700.017.1318151
AGCTTGA40450.017.083661
CGATTGA48450.017.0807131
ATGCGAT43650.017.0101741
TCGGTAA52850.016.9900511
CTGATAG40000.016.9870471
GGGACCT35800.016.9264681
Produced by FastQC (version 0.11.5)