FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12630092
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG42550.018.5602041
CGCTGGA45650.018.3810561
GGATTAC41800.017.9385131
CTGCCTA50450.017.9228251
GTTTGAC40000.017.7182398
CAATGGA50000.017.639498
TGATTTC46750.017.634071
TGACTAG36900.017.503031
GGGACCT28150.017.3848921
CCACTAC42000.017.3281
GACTAAC35700.017.3265291
CTTTGCG46600.017.1950361
ACTGAAC28250.017.1746541
GGCGACT33450.017.1733761
GGGAATG31950.017.092161
AGAAACC45150.016.9538
GAATGAA34950.016.947141
CGCTATC34050.016.9016041
GGCTCGA44500.016.850111
TTTGCGA25800.016.8493522
Produced by FastQC (version 0.11.5)