FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17777320
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTTC65700.018.1906491
CAATGGA71600.018.1544748
CCACTAC66800.017.8911041
CGCTGGA63800.017.8764171
ACTGATG60450.017.8594531
GACTAAC46450.017.4543191
CTGCCTA75650.017.450091
GGATTAC56650.017.4075241
AGAAACC63300.017.3501828
ACTGAAC41800.017.3106861
CTCGAAA43600.017.2704641
CTTTGCG66750.017.259441
GGCGACT46650.017.1543661
GTTTGAC58950.017.045288
AGACGTT56700.017.0402571
TTTGCGA35750.017.0063672
GGCTCGA59200.016.9947641
GGGAATG50650.016.9192871
GGGACCT40100.016.8922181
CGCTATC52500.016.8831881
Produced by FastQC (version 0.11.5)