FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505208
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA57350.018.0738411
CTGCCTA70000.017.94321
TGATTTC64500.017.8124791
CCACTAC58750.017.714651
ACTGATG55450.017.6893581
CAATGGA66500.017.6836688
GGATTAC52900.017.450181
GACTAAC43450.017.378241
ACTGAAC40900.017.3062651
GTTTGAC56950.017.2935878
GGGAATG43500.017.2858411
CTTTGCG58850.017.2205731
CGCTTCA43700.017.086571
TGACTAG47900.017.0792471
GAATGAA41000.017.059141
ATAACGC47900.016.9696241
TTTGCGA31500.016.9672762
CTGCGGA35100.016.9047241
TCTATTG56550.016.8809931
CGATTGA39450.016.8509141
Produced by FastQC (version 0.11.5)