FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16185058
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG54050.018.1282121
CTGCCTA68950.018.1099241
CAATGGA68200.018.1048158
CGCTGGA56250.017.997971
CCACTAC61250.017.7975541
TGATTTC59800.017.7549321
GGGACCT35800.017.4836581
GGATTAC51900.017.4829791
GACTAAC43150.017.3774641
GGCGACT40300.017.2252461
GTTTGAC52250.017.2212898
CGCTTCA43100.017.0564961
TTTGCGA34800.017.0484372
CTCGAAA38600.017.0316261
CTTTGCG63100.016.9595321
ACTGAAC37200.016.9385951
CTAATGG50150.016.8994141
GGGAATG39900.016.8451981
GATGCTA33450.016.7340151
TGCGTGG58250.016.7309681
Produced by FastQC (version 0.11.5)