FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15552977
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGGA59300.018.133641
TGATTTC62800.018.0092581
GACTAAC56800.017.9334221
TTTATGC52300.017.7095151
CGCTATC57750.017.5838621
ACTGAAC41400.017.5527651
CAATGGA51500.017.5335378
CCGGTAG51950.017.5256161
CTCGAAA42900.017.4775541
TACTTGT73550.017.3045251
CGATTGA43250.017.2875581
CGGACTG40100.017.2314281
CTGATCC59450.017.2223551
GGGAATG39900.017.159891
GGGAGAT42300.017.0303481
CTGCGGA36100.017.0172141
ACCCACT58300.016.985681
TGCTACC43600.016.9320131
CCGTGGA42400.016.8911131
GATGCTA34450.016.8873041
Produced by FastQC (version 0.11.5)