FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15895732
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC62200.018.437451
TGATTTC60450.018.1373061
TTTATGC57400.017.8569181
ACTGAAC44500.017.817861
TACTTGT72500.017.802561
CGGACTG39000.017.7724761
CAATGGA54150.017.66418
CCACGGA61600.017.6623151
CAATGAA36150.017.4847668
CCGTGGA44400.017.4795441
CTGATCC58700.017.461451
TGCTACC41600.017.3937991
CGCTTCA49750.017.3096941
CCGGTAG53900.017.1849561
GGGAGAT47050.017.1646651
GAACCTA52850.017.1289581
ATAACGC45750.017.1244371
ACTGATG33250.017.0557461
ACCCACT56550.017.0296361
GGCGACT39800.017.0194381
Produced by FastQC (version 0.11.5)