FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20022893
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC76500.018.5331041
ACTGAAC53000.018.0318951
TTTATGC74650.017.979471
TGATTTC81250.017.6822911
CGCTTCA64200.017.6016371
CCACGGA78200.017.5930181
CCGTGGA54200.017.5745331
CGCTATC69750.017.5561871
CTGATCC79750.017.4486181
CAATGGA66700.017.3785638
CTCGAAA57150.017.3105351
GGGAGAT55850.017.2621691
CGGACTG50750.017.2379171
GAACCTA61050.017.2196461
TACTTGT85950.017.1063751
TGACTAG53250.017.0597361
GGGAATG48750.016.9972321
AGCGTAT74400.016.9670891
CGATTGA52850.016.9305251
TGACGGC41200.016.9257031
Produced by FastQC (version 0.11.5)