FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_cDNA_1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_cDNA_1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18106704
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC67700.018.6154141
TGATTTC72250.017.8646351
TTTATGC60800.017.7051831
CGCTTCA57300.017.6686171
CAATGGA61000.017.625738
TGCTACC52700.017.6165871
ACTGAAC49750.017.605691
CCACGGA69350.017.5970481
TACTTGT78650.017.532591
CGGACTG43100.017.4224361
ATAGACC43200.017.1876221
CGCTATC61600.017.185411
TGGACGC44150.017.1745991
CCGTGGA51550.017.17431
CCGGTAG61700.017.1575581
GGGAATG49250.017.1233771
TGACTAG48350.017.0945781
ATAACGC53750.017.0770591
GGATTAC43050.017.076741
CTCGAAA51800.017.0508631
Produced by FastQC (version 0.11.5)