FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_BLG_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_BLG_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9908735
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCCC511750.020.7785991
ACATATG409800.020.6315567
CTCCCTC503600.020.571633
ACTCCCT504550.020.5559962
CCTCTTC504700.020.5208636
ATCAGAC414500.020.4719052
CATATGC414900.020.4702478
TTCTGGC509300.020.465210
GACATAT412300.020.446266
ATATGCT415650.020.4307829
TCTTCTG513550.020.3305938
CCCTCTT508050.020.3275785
TCCCTCT508350.020.3257034
CATCAGA436850.020.2566781
CTTCTGG516350.020.2081479
TCAGACA418650.020.1659323
CTCTTCT515150.020.1281367
TATGCTG421550.020.1199310
ATGCTGC110550.019.99250411
AGACATA419450.019.9924855
Produced by FastQC (version 0.11.5)