FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_BLG_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_BLG_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9179020
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTCA312850.020.3697014
TTACTTC314200.020.27553
CTTCAGG311450.020.2694266
CAGGCCC317800.020.2625649
AGGCCCA318000.020.22351610
TCAGGCC320150.020.1925418
ACTTCAG316050.020.0638985
GGCCCAA95250.020.06245611
GGCCCAC123250.020.05382311
TTCAGGC318150.019.9836187
TGGTGTG126400.019.937374
CTTACTT320150.019.8955062
TGTAATG125800.019.8141489
GTAATGA126850.019.59230210
ACTTACT342350.019.5893081
GTGTGTA145700.019.4587546
TAGTGGT140450.019.41081
CATGGAG44600.019.3997463
AGTGGTG127550.019.3954962
GGCCCAG65750.019.37058411
Produced by FastQC (version 0.11.5)