FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_BLG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_BLG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9903706
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA2280500.020.671768
AGTCGAA2278550.020.6567127
AAGTCGA2276950.020.6486636
TCGAAAG2284900.020.6305779
CAAGTCG2277950.020.620135
GCAAGTC2280750.020.6172734
CGAAAGC2290350.020.59294510
AGCAAGT2302400.020.5056463
GAAAGCG566450.020.4371711
GAAAGCA778100.020.38213311
GAAAGCC432500.020.36518911
TCTGCGT321350.020.352188
CGTAGCG326950.020.343631
GCGTCTG317650.020.3278925
CTGCGTA322000.020.3176179
GTCTGCG320150.020.2986157
GAAAGCT544050.020.20369311
TGCGTAA323700.020.1979410
TAGCGTC320250.020.1755663
GACTCGC162150.020.08610710
Produced by FastQC (version 0.11.5)