FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_BLG_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_BLG_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11324578
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGGG392100.020.32960510
GATCGTA407950.020.2921261
CTTTAGG393450.020.2625189
CGTATCT396400.020.1884754
TGCCCAC185600.020.1587244
CCCACGG186150.020.1555756
ATCGTAT400050.020.151362
ATCTTTA395450.020.0893297
TCTTTAG405000.019.9853888
TCGTATC403300.019.973263
CTGCCCA190550.019.9601923
GCCCACG189750.019.7675535
TCTGCCC193550.019.6997262
CCACGGC193350.019.6478527
TTAGGGC99800.019.62076411
CACGGCT196850.019.5482628
TATCTTT403250.019.5460686
CGGCTAC197300.019.4770710
TTAGGGG75550.019.41465211
ATCTGCC216800.019.3678571
Produced by FastQC (version 0.11.5)