FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_ADT_three.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_ADT_three.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4787804
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGG33200.019.1675326
AGGATGC20150.019.0718298
CACTCCG34050.018.9662115
AGCCCTA11550.018.727148
TTAACTC37050.018.650761
CTCCGGC27100.018.6373757
ACACTCC35600.018.5828844
CCTTCGA31700.018.5187381
CCGGCAC29250.018.487059
GTAAACC15200.018.443958
TGAGCCC10850.018.388896
AACGTAC19250.018.3832265
AAACCTC15200.018.3746810
GGATGCG20950.018.3435489
ACTGCCA12600.018.3332049
CGAGCAC19050.018.3021431
ACCTGTA17050.018.2301354
TGAGCAT23750.018.1734351
CCCGATA8900.018.1693612
CTGCGGA15850.018.1543251
Produced by FastQC (version 0.11.5)