FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_ADT_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_ADT_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115179
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAA49500.019.6858565
TTAGAGA47650.019.5027644
CATATCC17850.019.4717754
GAACAAT53500.019.4488169
ATATCCA17450.019.135655
AGAACAA56800.019.0952478
AGAGAAC50000.019.0479766
AACAATC54800.018.9874410
CCCATTA10850.018.9670459
GTTCTAC33750.018.760966
CCCGATA8800.018.734282
ATAGACC17900.018.716361
AAATGCC18400.018.6073061
CTGATCC27000.018.592931
TCGAGCC19400.018.5637518
TGTTCTA33400.018.5174145
TAGGCTG23100.018.499329
GGGACCT11450.018.3445991
GATGCCC11050.018.33869210
ACATATC19800.018.296573
Produced by FastQC (version 0.11.5)