FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_ADT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_ADT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11449137
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA94650.019.75676710
GTACGCG95800.019.5195189
ATAGACC72200.019.4326881
AGAAACC66850.019.3815368
CAGAAAC57500.019.3563697
CCCAAAG56550.019.2742376
ACGTTGA99050.019.2306232
ACCCAAA55050.019.2081095
AACGTTG114500.019.2059081
TAGACCC58700.019.1942942
GACCCAA55350.019.1797984
CGTTGAG99750.019.085063
GAGTACG101050.019.0047997
ACCCACT54400.018.8800681
AGTACGC107800.018.875988
GCAGAAA55150.018.6020776
TATGACA47100.018.5693510
TATGCCC27600.018.4922941
CATATTC52400.018.4583111
AAACCAT59300.018.37872110
Produced by FastQC (version 0.11.5)