FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_ADT_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_ADT_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17681066
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTGC192500.020.0136435
CGGTGCA192600.019.9269816
GACTGCG219900.019.8536971
ACTGCGG196650.019.7407972
GTGCACC252600.019.577628
TGCACCA252500.019.5728449
GCACCAC252250.019.5715410
GCGCCTC82450.019.369119
CCCCATC29050.019.2297955
AGATGCC80750.019.2048238
CAGCCGA173650.019.0149141
ATTGAGA66700.018.9701354
AAATGCC114550.018.9509931
TGCGGTG204300.018.9502074
CTGCGGT204950.018.9464113
AGCGCCT93750.018.8936618
TATTGAG81450.018.886633
GATCAGC151800.018.8013086
GGTGCAC261000.018.7391437
GATGCCT72150.018.5688699
Produced by FastQC (version 0.11.5)