FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_4HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_4HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585105
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAAAT264100.020.7939832
GATGAAA270000.020.782231
TCTTGCC267800.020.6582558
GAAATCT265150.020.652464
CTTGCCG268500.020.6004879
AATCTTG266500.020.5793656
ATCTTGC266050.020.5784287
AAATCTT266100.020.5668955
TGAAATC268500.020.4417173
ACAGGAC94500.020.2009226
CAAACAG100350.020.1952443
GAAGATC61000.020.0886216
TTGCCGT275250.020.08363310
AAACAGG94950.020.0498894
TGAAGAT60500.020.0290155
ACAAACA101650.019.9367492
CAATGGA27050.019.834888
AACAGGA96550.019.7720075
TGCCGTT91700.019.7510911
GGACAGC99600.019.6183769
Produced by FastQC (version 0.11.5)