FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_4GDO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_4GDO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12920928
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC48100.018.9956511
AGCTTGA49100.018.8654441
ACGAGGA75200.018.7419451
CAATGGA53450.018.6225038
CTTAGGA55300.018.5734981
ACTGATG40800.018.4303191
ATAGACC35450.018.426981
GAGGATC57500.018.2616443
ACTAGGA39400.018.255855
TGACTAG45350.018.2254161
GCAGTTA88950.018.2179681
ATGCGAT52050.018.1997661
CTGATGA28300.018.1439482
GGCCGAA38350.018.069919
GTGATAA45600.018.052148
CTCGAAA27100.018.0203691
GCGTTGA33650.017.9118166
TCGAATG40850.017.8636478
CTGAAAC39650.017.8259161
GGTATTG48650.017.8094731
Produced by FastQC (version 0.11.5)