FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_3d_cDNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_3d_cDNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13882330
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG44850.018.6154651
GATGCTA44750.017.7887481
TACGGAT63550.017.7483481
TGCTACC51900.017.6448651
TTTATGC50450.017.631591
TATGCCC47750.017.5948621
GGGAATG45950.017.552741
GATGAAA41400.017.5285871
GAACCTA48600.017.3951761
GACTAAC39250.017.3649791
CAATGGA41600.017.3395888
GGATTAC44300.017.3056681
TGACGGC35700.017.29731
TGGACGC40950.017.285231
AGCTACC33300.017.2787258
CGACCTT60350.017.2624991
AGATTGC50150.017.2344781
GCGCGAT33200.017.2079871
ACTGAAC34850.017.1767621
TACACGA50200.017.1545521
Produced by FastQC (version 0.11.5)