FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_3c_cDNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_3c_cDNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12824655
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC40150.018.6761131
ACTGATG43200.018.0868511
GATGAAA39200.018.0035041
GATGCTA41550.017.8193511
TACGGAT54550.017.7697281
TGCTACC47750.017.7270091
TGACTAG35200.017.6923621
TATGCCC43750.017.6194291
TGACGGC30750.017.5546361
TTTATGC45600.017.4803751
GGGAATG43150.017.4506721
GGATTAC43100.017.4465481
ATTACAG55200.017.3484293
ACTGAAC29800.017.3389431
CGACCTT51650.017.3237971
CCTTCGA52950.017.2951491
TATGACA34300.017.1424510
TGATTTC33450.017.142351
GTCGACT31400.017.120488
GACTGCG51850.017.1151921
Produced by FastQC (version 0.11.5)