FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_3b_cDNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_3b_cDNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22435979
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG73750.018.1133961
GATGCTA74450.017.9430891
GACTAAC68200.017.893551
TGCTACC82050.017.8298341
TATGCCC79750.017.790961
GATGAAA69500.017.7704031
GAACCTA72050.017.6370581
TACGGAT98050.017.512371
TTTATGC79100.017.4990181
GGATTAC73600.017.4796621
GTCGACT53750.017.463348
TGACGGC58650.017.4049591
TACACGA80600.017.3688011
GGGAATG79650.017.3651
CAATGGA69150.017.3397258
TGGACGC61550.017.3356631
ACTGAAC53750.017.3308531
TATGACA63550.017.29817810
CCTTCGA93450.017.28431
GGGACCT53400.017.1101071
Produced by FastQC (version 0.11.5)