FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_3a_cDNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_3a_cDNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13083501
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC33500.018.0563031
GACTAAC40350.018.0360281
CGACCTT51900.017.8464741
ACTGATG43350.017.829531
GATGCTA42650.017.8020691
GGATTAC43850.017.6501771
TTTATGC49700.017.622231
CTGATAG41800.017.5862371
TGCTACC48100.017.575271
TGACTAG36100.017.5703721
GGGAATG44300.017.5657081
CAATGGA38600.017.436088
GATGAAA39500.017.4138741
CGCTGGA39250.017.283991
GAACCTA41300.017.2651731
CCTTCGA54200.017.2635291
TATGCCC44950.017.2416111
TATGACA35450.017.23798610
AGCTACC29950.017.178198
GCGCGAT31700.017.1270471
Produced by FastQC (version 0.11.5)