FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_3HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_3HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4993975
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGTG131900.020.3950632
GTCGGCA134600.020.2275778
GGCTGGT138450.020.1992021
CTGTAAG78600.020.145516
CCCGATA21500.020.1209982
CGGCACT132600.020.12090710
CGTCTTC141350.020.10103210
ACGATGT148200.020.0395151
GTGTCGG131700.020.0357746
TGTCGGC132300.020.015947
TGTCGTC141350.020.0120947
TCTGTAA77650.019.9996225
CGATGTG141700.019.9923042
TCGGCAC134050.019.9659239
CTGGTGT140150.019.9212233
ATGTGTC141550.019.9172574
TGTGTCG141850.019.9047435
GATGTGT142550.019.8657263
GTGTCGT142700.019.7643036
GTCGTCT144700.019.7227448
Produced by FastQC (version 0.11.5)