FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_3GDO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_3GDO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11017302
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG54000.018.865111
AGCTTGA38150.018.802151
TGCTACC52550.018.5662571
GATGAAA47800.018.565651
TATGCCC49950.018.418321
ACTGATG39550.018.4021321
GAACCTA51450.018.3916551
GGATTAC37350.018.3613221
CTTGGCT62700.018.2742081
GATGCTA34400.018.2262481
TCGAGGA32300.018.2055512
CTCGAGG48050.018.1849171
GGCCGTC29900.018.121612
GACCTGG46700.018.103411
TTTATGC45600.018.056461
ATAACGC41650.018.0542491
AAGACCT55400.017.971321
TGGACGC43300.017.9483781
CCTACCA43150.017.9134161
GACTAAC33900.017.8754711
Produced by FastQC (version 0.11.5)