FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_2HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_2HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4718242
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAC14550.019.8493481
TACGAAA26300.019.7217969
GGTACGA25600.019.4822067
TCTTGTA40450.019.389988
GGCCACT43100.019.2952672
ACCCACT20650.019.2750591
GCCACTC43100.019.2706993
TAACCAA25650.019.1994324
GTAACCA27000.019.094843
GATGCTA24150.019.0907921
GTACGAA27250.019.0727798
GTCCACC21400.018.9889135
CCGCTAG25350.018.969810
TTGTATC35800.018.94631810
AACCCAT7300.018.84185610
AGGCCAC53650.018.831371
ATGCTAC19750.018.6613432
TTTCTTG35750.018.592196
GAACCTA22150.018.5861261
CATTTTC36850.018.5784033
Produced by FastQC (version 0.11.5)