FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_2GDO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_2GDO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12153683
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATGA52600.019.088011
GACTAAC49600.018.9085541
TGATTTC61750.018.8788071
CTGCCTA56950.018.7549441
TATGACA50500.018.5252510
CTATTGA33250.018.4744832
AAACCTG48000.018.3573191
CGCTGGA39400.018.2325921
TATTGAG35550.018.2243423
AGCTTGA39950.018.0604461
TCTATTG51700.018.059251
CGCTTCA45000.018.0174481
CCACTAC41700.017.957341
GGATTAC42650.017.9513451
GATGCTA28200.017.9509091
GGAACTT46200.017.8677181
ATAGACC39500.017.8141961
GTATGAC52600.017.7855749
CTGCGGA33300.017.756311
CAATGGA49600.017.7393028
Produced by FastQC (version 0.11.5)