FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_1HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_1HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5063068
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAG1149350.020.9143851
TAAGAGG1153250.020.7586562
AAGAGGT1158900.020.6565443
GAGGTCC1160350.020.5038385
AGAGGTC1164600.020.4813044
CCGCTGA1161500.020.454510
GTCCGCT1163900.020.4427958
TCCGCTG1163500.020.4362859
GGTCCGC1162800.020.435657
CGCTGAA330850.020.35219411
AGGTCCG1170050.020.3132136
CGCTGAG318250.020.24738111
CGCTGAC269600.020.20121611
CGCTGAT256950.020.09652711
GCTGACC72750.019.98931512
GCTGATC65200.019.95284712
GCTGAGC87600.019.9088712
GCTGAAG89700.019.85246812
GCTGATG64650.019.83025212
GCTGACG73250.019.80986812
Produced by FastQC (version 0.11.5)