FastQCFastQC Report
Wed 24 Apr 2019
HCG37BGXB_n01_1GDO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG37BGXB_n01_1GDO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15434326
Sequences flagged as poor quality0
Sequence length27
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG79400.019.3376081
GACTAAC69350.018.9446561
CGGACTG55950.018.9394321
CGCTTCA71950.018.654171
TACTTGT99550.018.5988851
CCCATAT22500.018.574456
CTGAAAC52600.018.4485441
CCACGGA74750.018.4471551
ACCCACT70200.018.3113421
TGCCAAA79300.018.2495381
AGCCTAA75850.018.0827081
TGATTTC68200.018.0783821
TACTAGA42550.018.0397434
GCCTCTA78200.018.0362031
TGACTAG47050.018.0131531
AACAACC52600.017.9852479
TGCTACC58550.017.9549031
GGCTCGA62150.017.8780611
TAGTTGG54700.017.8746781
GGAGATA49050.017.8539352
Produced by FastQC (version 0.11.5)