FastQCFastQC Report
Sat 30 Apr 2022
HCG25DRX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCG25DRX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125107039
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG5000760.39971851623792326Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4893350.3911330680602232No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA4838830.3867751997551473Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC2061660.16479168690100643Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG1644920.13148101123230962Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGGTGAGATGGCTCAGTG1528650.1221873694892579Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTG1280340.10233956540207143Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)