FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12983781
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC138100.10636346993221774TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT447350.064.6621312
GCACACG446700.064.6533911
ACACGTC438200.064.3626513
ACGTCTG435400.064.24328615
CACGTCT435150.064.2131414
CGTCTGA434600.064.18104616
GAACTCC443450.063.21985221
AGCACAC464000.062.9823210
CTGAACT446000.062.66609219
AGAGCAC481500.062.406558
TGAACTC455650.062.18861420
CTCCAGT428150.061.99914624
CCAGTCA392050.061.07567226
CGGAAGA505350.061.048014
TCGGAAG504450.060.8671883
GATCGGA504450.060.6848141
CAGTCAC348300.060.6766127
AACTCCA465300.060.6139922
ATCGGAA519400.059.3345152
AGTCACC130800.058.66644728
Produced by FastQC (version 0.11.5)