FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_BR04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_BR04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8448213
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC92204610.914095087327935TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC992700.067.7504331
GCACACG1004850.067.6579611
ACGTCTG1002800.067.651315
CGTATGC987150.067.6492344
ACTAGCT992250.067.6075232
GTATGCC989150.067.60210445
CACGTCT1004050.067.5976414
GCCGTCT985700.067.572849
ACACGTC1006000.067.5505113
TATGCCG989550.067.5146446
CGTCTGA1005050.067.4723816
ATGCCGT989400.067.46447
TGCCGTC989100.067.410948
CACACGT1008550.067.4062812
CTCGTAT989150.067.3846642
TCGTATG992250.067.26977543
TCACTAG1001950.067.2398230
TAGCTTA995000.067.22369434
ACTCCAG1002600.067.1520523
GCTTATC991150.067.1509636
Produced by FastQC (version 0.11.5)