FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_BR03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_BR03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30067319
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC12298794.090417905234584TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1339650.066.8420744
TATGCCG1345150.066.5713746
GTATGCC1347100.066.4727445
ATGCCGT1348000.066.40996647
TGCCGTC1356300.066.0176248
AGTCACG1357400.065.9621428
GCCGTCT1359400.065.85676649
CTCGTAT1356450.065.77875542
TCGTATG1363100.065.70466643
GATCTCG1364650.065.18394539
CACGATC1375750.064.9496131
CGATCAG1374450.064.8479733
GTCACGA1380500.064.7844429
CCGTCTT1389350.064.43973550
CAGATCT1381750.064.23730537
CAGTCAC1395950.064.2130527
CCAGTCA1398100.064.0372426
CTGCTTG1401900.063.90211557
AGATCTC1393500.063.7385638
TCTCGTA1403300.063.52314841
Produced by FastQC (version 0.11.5)