FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_BR02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_BR02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29722843
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC426699814.355955115060832TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4558100.068.1015644
GTATGCC4566100.068.0787245
TATGCCG4564250.068.0273346
TCGTATG4557750.067.9505743
CTCGTAT4547750.067.9358442
GCCGTCT4555050.067.8448949
ATGCCGT4572800.067.8418947
TGCCGTC4577100.067.67992448
GTCACAC4658000.067.5453629
CCGTCTT4589400.067.5201350
CAGTCAC4681150.067.4524827
TCTCGTA4593550.067.4430941
CCAGTCA4678750.067.43246526
CTGCTTG4561500.067.3997457
ACTCCAG4687500.067.3296223
TCCAGTC4684700.067.32078625
AGTCACA4693750.067.1862228
GAACTCC4715800.067.1345921
ATCTCGT4618650.067.1220440
AATCTCG4617950.067.0668139
Produced by FastQC (version 0.11.5)