FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_BR01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_BR01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27460803
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC276069910.05323478705266TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3063450.067.50403644
GTATGCC3064700.067.4931145
TATGCCG3067850.067.43407446
CTCGTAT3061850.067.3537642
TCGTATG3069700.067.2831343
ATGCCGT3075850.067.2294747
GCCGTCT3069800.067.19794549
AGTCACC3082600.067.07490528
TGCCGTC3082800.067.0336648
CCGTCTT3089950.066.7993850
CAGTCAC3105700.066.7048727
TCTCGTA3094600.066.6185841
CTGCTTG3089150.066.5946157
CCAGTCA3102750.066.5552326
GTCACCA3106050.066.508329
CATCTCG3082300.066.5059839
ACTCCAG3111150.066.3989123
ACCAGAT3113000.066.3945832
TCCAGTC3113250.066.3217625
GAACTCC3128500.066.2340421
Produced by FastQC (version 0.11.5)