FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_AKM65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_AKM65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31057573
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5122351.6493078837808737TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC587200.064.3374744
GTATGCC595350.063.4513645
TATGCCG597550.063.264246
GTCACGC594500.063.1571729
ATGCCGT601100.062.8855647
TCGTATG602300.062.73010343
GCCGTCT603200.062.65971849
CTCGTAT602850.062.5799842
TGCCGTC605700.062.3546648
AGTCACG606300.062.2859828
TCACGCC601200.062.18522630
CACGCCA603300.061.81793231
CCGTCTT637200.059.34365550
ACGCCAA630850.059.1179932
CTGCTTG642300.058.79720757
CAGTCAC644800.058.7564827
CCAGTCA645900.058.4835926
CGCCAAT640950.058.10441233
TATCTCG649300.058.0804139
TCTCGTA654800.057.69007541
Produced by FastQC (version 0.11.5)