FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_AKM64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_AKM64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32657675
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3508381.0742895812393258TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC435000.060.9327344
TATGCCG437900.060.59295746
GTATGCC440950.060.1181945
ATGCCGT443800.059.8037447
GCCGTCT449700.058.9094449
TGCCGTC450600.058.85364548
CTCGTAT451500.058.3723542
TCGTATG457150.057.8731243
GATCTCG467750.056.17879539
CACAGTG478350.054.96930733
CCGTCTT486800.054.5421550
GTCACAC489050.053.77353729
CTGCTTG491850.053.69815457
ACACAGT502150.052.53124632
CACACAG501100.052.48774331
AGTGATC500500.052.4324336
TCTCGTA505800.052.2102441
CAGTCAC517000.051.11667627
CCAGTCA519700.050.66293726
ACAGTGA525450.050.02224334
Produced by FastQC (version 0.11.5)