FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_AKM63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_AKM63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34975676
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2278090.6513355167173895TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC310050.056.21807544
TATGCCG316100.055.141746
ATGCCGT319100.054.5578747
GTATGCC321800.054.16490645
TGCCGTC326900.053.2756748
GCCGTCT328350.052.93419349
CTCGTAT331400.052.16290742
TCGTATG336350.051.9052143
ACTGACC354950.048.12701832
CTGACCA356100.047.86355633
CCGTCTT370550.047.06649850
CTGCTTG379800.045.79249657
CAGTCAC387000.045.00922427
CCAGTCA386750.044.8666426
TCTCGTA386400.044.8287141
GTCACTG391950.044.15550229
GACCAAT389100.043.7952635
CACTGAC395350.043.6694131
CCAATCT390450.043.4826837
TCCAGTC408450.042.4829825
Produced by FastQC (version 0.11.5)