FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_AKM62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_AKM62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26879836
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC20571647.653186574501422TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2226100.067.64831545
CGTATGC2230250.067.53367644
ACTTAGG2229850.067.4876532
CACTTAG2234500.067.4586731
CTCGTAT2226000.067.4012242
TATGCCG2235750.067.37668646
TCGTATG2235800.067.34869443
GCCGTCT2245950.066.9902749
AGGCATC2230650.066.9395336
ATGCCGT2254800.066.8101747
GGCATCT2235550.066.73801437
CCGTCTT2256850.066.66518450
TTAGGCA2253750.066.6005334
TCTCGTA2250400.066.5590641
TGCCGTC2270250.066.3378648
TAGGCAT2258050.066.31480435
GCATCTC2252000.066.3052838
CAGTCAC2284850.066.14281527
CTGCTTG2273200.066.0862957
CATCTCG2264100.066.01980639
Produced by FastQC (version 0.11.5)