FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_AKM61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_AKM61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28415103
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC324890011.433708334613463TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3525000.068.1240413
ACGTCTG3517100.068.089115
CGTATGC3482300.068.0854144
GTATGCC3487500.068.00597445
CACGTCT3524850.067.99377414
GCACACG3533400.067.9857411
CACACGT3535300.067.93732512
TATGCCG3491400.067.9357846
ATGCCGT3493900.067.8644947
GTCACCG3488400.067.85152429
AGTCACC3497550.067.8223528
CTCGTAT3489500.067.8108142
TCGTATG3496100.067.807343
GCCGTCT3493000.067.7888949
CGTCTGA3533750.067.7705116
TGCCGTC3498800.067.7284148
CGATGTA3481250.067.71641534
ACTCCAG3514050.067.6388723
CCGATGT3487100.067.6121433
GTATCTC3487900.067.59280438
Produced by FastQC (version 0.11.5)