FastQCFastQC Report
Thu 13 Feb 2020
HCFFFBGXF_n01_AKM60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCFFFBGXF_n01_AKM60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26895587
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC22272928.281254467507997TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2437100.067.9463411
ACACGTC2438200.067.8906113
ACGTCTG2435400.067.7832715
CACGTCT2439850.067.7054314
CACACGT2446250.067.68490612
CGTATGC2425050.067.62694544
CGTCTGA2443250.067.5700416
GATCGGA2458200.067.534361
TATGCCG2427950.067.493946
GTATGCC2428750.067.48344445
ATGCCGT2433550.067.3175147
CTCGTAT2425200.067.27119442
GCCGTCT2434150.067.26884549
TCGTATG2439650.067.2234243
TGCCGTC2437900.067.1739848
CACGATC2401450.067.1716236
AGCACAC2474900.067.0491110
ACGATCT2411500.066.9769437
CGATCTC2420200.066.8681438
TCGGAAG2487950.066.846753
Produced by FastQC (version 0.11.5)