FastQCFastQC Report
Thu 13 Feb 2020
HCF7YBGXF_n02_Salt_NSF_Plate_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCF7YBGXF_n02_Salt_NSF_Plate_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383453939
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50664901.3212773386062413No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT5347090.13944543154112704No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5136300.13394829150522822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA924250.021.8998471
GTATCAA3153800.020.6493031
GGACCAT2633400.019.1130586
GTTATAC1912900.017.692473
TGGACCA3094300.017.4599785
ATACCGG1902050.017.1318156
GTACTGG2290300.016.8082031
TAGTCCA847900.016.7082799
TTAGTCC827750.016.6178768
GGGGGTA1908750.016.59087821
TCAGGGG1895450.016.49910718
ACTGGAC2240850.016.4537833
TACCGGT2064400.016.1421497
GGGGTAT1944200.015.96343922
GGTATTC1925850.015.93263424
TACTGGA2365500.015.9154482
TATACCG2187450.015.8967625
CAGGGGG1971550.015.884319
GGGTATT1958150.015.81710523
TTGGACC1090800.015.742234
Produced by FastQC (version 0.11.5)