FastQCFastQC Report
Thu 13 Feb 2020
HCF7YBGXF_n01_Salt_NSF_Plate_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCF7YBGXF_n01_Salt_NSF_Plate_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383453939
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG68725491.7922749777777092No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCACC4557500.013.8898422
CCTCTCT3862950.013.8602624
ACACCTT4473900.013.8585092
TACACCT4492500.013.8246891
CTCCTAC3061250.013.8212064
CCTCAGT4002000.013.8150996
TCCTTAC3229750.013.80954
CCACACT3085050.013.8049172
AATCCTT3224050.013.7959892
TCGACCA3022300.013.7913532
TCCACCT4601650.013.7865583
AACTACC2800600.013.7796056
CGCATCC3077150.013.7777775
CCTACGT3067600.013.7754936
TCGACTT3236750.013.7748896
TCCTGGT4721300.013.77251155
CCACCTG4604200.013.7723494
AGACGTC4856250.013.7633381
AAGCGCT3894050.013.7625463
CACCTTC4504250.013.7611233
Produced by FastQC (version 0.11.5)