FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16474334
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169159910.268087316913691No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN180600.1096250689102212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG44500.07.455059570
CTAGGTG5450.00170186277.048288370
GTAGATA14951.8189894E-127.007571770
TCTCGGT51500.06.309036369
GTGTAGA92850.06.13046170
AAGGGGG18851.8189894E-126.116353568
CGCCGTA20453.6379788E-125.813297357
GCCGTAT20453.6379788E-125.813208658
TGTAGAT91350.05.81062170
AGTGTAG102800.05.77754269
TAGGGGG9103.023107E-45.76431538
AAAGGGG19956.91216E-115.605045367
TAGATCT68100.05.538142770
GATCTCG60900.05.507332369
TCGGTGG44850.05.45033370
GAGTGTA110000.05.42863870
AGATCTC69300.05.240679370
GTAGATC93500.05.19147470
ATGGGGG15104.2751144E-65.090178569
CGTATCA22955.838956E-105.027517360
Produced by FastQC (version 0.11.5)