FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n02_B.F1.mbWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n02_B.F1.mbWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31884932
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG167050.010.66854870
AAGGGGG153600.09.11795369
CTCGGTG344550.08.19062170
TAGGGGG57100.07.78707538
TCGGTGG321650.07.56545970
AAAGGGG189900.07.337828668
CGGTGGT254900.07.156520470
TAGATCT557750.06.880262470
ATCTCGG452950.06.81792770
TCTCGGT428450.06.75007369
GATCTCG527650.06.5892770
GTGTAGA795500.06.448095370
AGATCTC598850.06.197575670
TGTAGAT792550.06.18935770
GGTGGTC245900.06.065757870
AGTGTAG888650.06.04398269
GAGTGTA963050.05.68612669
CGCCGTA113550.05.641080456
GTAGATA80950.05.57964970
GTAGATC824300.05.53893570
Produced by FastQC (version 0.11.5)