FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n02_B.F1.fcWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n02_B.F1.fcWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41484493
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG115700.07.80736770
CTCGGTG543450.07.512028770
CGGTGGT420950.07.352585370
ATCTCGG708500.07.2544570
TAGATCT909200.07.01243570
GTAGATA122900.07.00811970
TCGGTGG522550.06.82754670
TCTCGGT661800.06.74530770
GATCTCG797400.06.673991770
AGATCTC918300.06.580736670
GTGTAGA1294900.06.337816770
TGTAGAT1277400.06.232780570
AGTGTAG1432900.05.983994570
GGTGGTC402200.05.69316770
GAGTGTA1553900.05.66441169
GTGGTCG367700.05.65602270
TGGTCGC285900.05.5598170
CGCCGTA191100.05.531480356
GCCGTAT198000.05.51558957
GTAGATC1337150.05.4252869
Produced by FastQC (version 0.11.5)