FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n02_B.F1.faWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n02_B.F1.faWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30189150
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307210.1017617256530906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG184500.014.80221569
AGGGGGG245000.013.70540370
AAAGGGG211600.012.85629668
TAGGGGG62700.011.89339938
AAAAGGG294150.09.70027767
ATGGGGG110800.08.81665270
CTCGGTG353100.08.60718870
TCGGTGG345550.07.99475970
TCTCGGT412350.07.64215470
GGGGGTA94200.07.542985453
ATCTCGG450900.07.446936670
TAGATCT572400.07.291483470
TTAGGGG45350.07.179600737
AAAAAGG467250.07.14033966
GGTGGTC273500.07.130782670
GGGGGTT169200.07.074853452
AAAAAAA2685550.06.889854462
CGGTGGT350150.06.869765370
CGGGGGG149850.06.771878212
AGATCTC554800.06.74653170
Produced by FastQC (version 0.11.5)