FastQCFastQC Report
Fri 6 Mar 2020
HCCYVBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCYVBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16474334
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170378910.342081203404033No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236540.14358091805107265No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG224440.13623615983505008No Hit
GGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216300.131295140671544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG43150.053.9247672
GTCGGGG31700.045.901571
AGGGGGG60150.027.9395561
CTCGGGG12600.027.6822511
CGGGGGG199150.024.3464783
CTGGGGG13950.023.487971
AGCGGGG10050.023.1374031
TGCGGGG13600.022.5380881
CGCGGGG13700.022.116411
GGGGAGG26150.020.7484551
ATGGGGG14600.020.2704411
ATCGGGG13450.019.1221771
GAGGGGG39450.017.2587852
GGAGGGG40450.017.2458021
GCGGGGG148100.017.1332112
GCTTGAA42550.014.71796961
ATGCCGT45900.013.73430249
CTCGTAT45350.013.42331544
GGCGGGG141350.012.2632621
TCTCGTA49400.012.0403443
Produced by FastQC (version 0.11.5)